Species:

Universal

Alternative Name:

Oestradiol; E2; 17β-Estradiol; Estra-1,3,5(10)-triene-3,17β-diol

Assay Type:

Competitive

unit:

pg/mL

Standard:

800pg/mL

Sensitivity:

7.5 pg/mL

Detection Range:

12.5-800 pg/mL

Assay Time:

1.5H

Sample Type:

Serum, Plasma, Other biological fluids,Sample volume: 50μL

Research:

Signalingtransduction；Cancer；Metabolism

Test Principle:

This ELISA kit uses the Competitive-ELISA principle. The micro ELISA plate provided in this kit has been pre-coated with E2. Standards or samples are added to the appropriate micro ELISA plate wells then with a biotin-conjugated antibody specific to E2. Next,Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of E2 in the samples is then determined by comparing the OD of the samples to the standard curve.